A diesel oil-degrading bacterium was isolated from sea waters. From the morphological and biochemical properaties of Gram-negative, aerobic, nonmotile, carbohydrate utilization and indole production, the isolate was tentatively identified as
Pseudononas
Y31. Our isolate showed a maked drug resistance against penicillin or ampicillin, whereas sensitive reactions were observed toward tetracycline, amikacin and moxalactam. In addition diesel oil-degrading capability(Die+) was examined for the
isolated
plasmid using mitomycin C, In the absence of related plasmid the cured cells could not grow on minimal medium containing the oil, this implied that the plasmid DNA might play and important role on the diesel oil degradation. Further, a plasmid
with the
property of Die+ was observed from the transformed E. coli cells, and the size appeared to be approximately 14 Kb.
|